Isolated bacterial strain of <i>Achromobacter </i>sp. MTCC 5605 and a highly enantioselective epoxide hydrolase isolated therefrom

摘要

The present invention relates to a novel epoxide hydrolase enzyme which aims to achieve a high degree of resolution towards a broader range of substrates with high enantioselectivity and yields with minimal product inhibition. The invention further relates to a new bacterial strain Achromobacter sp. MTCC 5605 isolated from a petroleum-contaminated sludge sample, capable of producing the said enzyme. It is notable that the enzyme can be used as whole bacterial cell preparation, which allows continuous hydrolysis of substrates at even higher concentration and have an advantage of being recycled. The invention further relates to a process for the hydrolysis of different aryl epoxides which are potential synthons of intermediates for the synthesis of chiral amino alcohols and bioactive compounds like β-blockers.

主权项

1. An aqueous composition of an isolated enantioselective epoxide hydrolase from Achromobacter MTCC 5605 having a molecular weight of 95 KDa, said enantiomeric epoxide hydrolase being active in the pH range of 5 to 11 and at a temperature ranging from 30° to 50° C., exhibiting hydrolysis of racemic aryl epoxides with >99% ees at a substrate concentration up to 500 mM, yielding up to 42% of 100% optically pure aryl epoxides with a final concentration of up to 209 mM wherein said composition is free of petroleum contamination sled′ e.