摘要 |
<p>The present invention aims to provide a method of hydrolysis of cellulose using a filamentous fungus-derived cellulase as a carbohydrase, which method comprises the step of adding the carbohydrase to cellulose to perform primary hydrolysis and then subjecting the primary hydrolysate to solid-liquid separation into a primary sugar liquid and solids; the step of adding water to the solids and performing secondary hydrolysis, followed by subjecting the secondary hydrolysate to solid-liquid separation into a secondary sugar liquid and a residue; and the step of filtering the primary sugar liquid and/or secondary sugar liquid through an ultrafiltration membrane, and recovering the carbohydrase from the feed side and recovering a sugar solution from the permeate side. By this, a method to reduce the amount of enzyme, such as cellulase, used in a method for producing a sugar liquid from pretreated cellulose is provided.</p> |