ACELLULAR VASCULAR PRODUCTS

摘要

A method of preparing a natural acellular vascular tissue that is immunologically inert and is free of epitopes capable of reacting with pre-formed human antibodies and also without the ability to activate complement. The method comprises the following methodological steps: (i) a pre-treatment incubation with 200mM ethylenediaminetetraacetic acid (EDTA); (ii) a first disinfection wash; (iii) at least two cycles of an incubation with a hypotonic buffer and an anionic detergent; (iv) a nuclease treatment; (v) a hypertonic wash; and (vi) a terminal sterilization process. The disclosure also includes products made by the method and uses of the acellular vascular tissue especially in bypass surgery.