AN IMPROVED RICE HAVING INCREASED LYSINE CONTENTS, AND METHOD OF PRODUCING THE SAME

摘要

The present invention relates to an endosperm specific lysine double construct (LDC) for increasing lysine contents in rice comprising cloning of: (i) cdapA overexpression cassettes (cdapA gene); and (ii) endosperm specific LKR/SDH RNAi cassettes (LKR/SDH RNAi construct), wherein the cloning is carried out under control of endosperm specific promoter GlutelinD1 in a same plant expression vector pCAMBIA1300, and wherein the cdapA over-expression cassettes or the cdapA gene is a corynebacterium dapA gene, and wherein the corynebacterium dapA gene is a dihydrodipicolinate synthase (DHDPS) gene from Corynebacterium glutamicum. The present invention also relates to an endosperm specific lysine double construct (LDC) pCAMBIA1300GltD1pcdapAGltD1pLKR/SDHRNAi construct; and an endosperm specific promoter GlutelinD1 comprising sequence listing of SEQ. ID 2. The present invention also relates to methods for: preparation of an endosperm specific lysine double construct (LDC) pCAMBIA1300GltD1pcdapAGltD1pLKR/SDHRNAi construct; increasing lysine contents in rice by employing the endosperm specific lysine double construct (LDC) of the present invention; transformation of lysine double construct (LDC) into Oryza sativa L. cv IR64 for increasing lysine contents in the rice by employing the endosperm specific lysine double construct (LDC) of the present invention; a method for developing rice plants or rice lines, wherein the rice has been unexpectedly found to have increased lysine contents. In one embodiment, the present invention also relates to rice having increased lysine contents; and rice having improved nutritional quality, i.e. economical value due to increased lysine contents. Figure 3