METHOD OF IDENTIFYING OR EVALUATING BENEFICIAL ACTIVES AND COMPOSITIONS CONTAINING THE SAME

摘要

A method of identifying or evaluating skin-care actives that improve the metabolism of a skin cell. The method includes contacting cells with a stressor and a test agent and determining a response of the cells to the stressor and test agent, based on the change in metabolic indicators associated with glycolysis and/or oxidative phosphylation. The test agent may be identified as a skin-care active when the oxidative phosphorylation and/or glycolysis response corresponds to an improvement in cellular metabolism.

主权项

1. A method of identifying a test agent as a skin-care active that improves the metabolism of keratinocytes, comprising:
a. providing a plurality of keratinocytes; b. exposing the keratinocytes to a stressor; c. non-lethally detecting a metabolic indicator associated with each of glycolysis and oxidative phosphorylation to provide a response of each to the stressor; d. exposing the plurality of keratinocytes to a test agent; e. non-lethally detecting the metabolic indicators associated with each of glycolysis and oxidative phosphorylation to provide a response of each to the test agent; and f. identifying the test agent as a skin-care active when at least one of the responses of (e) indicates an improvement in keratinocyte metabolism relative to the corresponding response of (c).