| 发明名称 |
METHOD FOR THE PRODUCTION OF POLYPEPTIDES IN THE PERIPLASM OF PROKARYOTIC CELLS |
| 摘要 |
Herein is reported a method for producing a polypeptide comprising the step of incubating (resuspended) prokaryotic cells in a solution comprising about 10 mM to about 95 mM Tris-HCl and about 2 mM to about 6 mM EDTA at a pH value of about 7 to about 10 for about 15 min to about 6 h at about 25° C. |
| 申请公布号 |
US2015275260(A1) |
申请公布日期 |
2015.10.01 |
| 申请号 |
US201514659232 |
申请日期 |
2015.03.16 |
| 申请人 |
Hoffmann-La Roche Inc. |
发明人 |
Schantz Christian |
| 分类号 |
C12P21/00;C07K14/21;C07K14/47 |
主分类号 |
C12P21/00 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method for producing a polypeptide comprising:
incubating prokaryotic cells in a solution comprising about 10 mM to about 95 mM Tris-HCl and about 2 mM to about 6 mM EDTA at a pH value of about 7 to about 10 for about 15 min to about 6 h at about 25° C., optionally wherein said prokaryotic cells are resuspended,wherein the solution is substantially free of sugar and peptidoglycan hydrolyzing enzymes. |
| 地址 |
Little Falls NJ US |
