ENGINEERING OF MULTI-CARBON SUBSTRATE UTILIZATION PATHWAYS IN METHANOTROPHIC BACTERIA

摘要

The present disclosure relates to genetically engineered methanotrophic bacteria with the capability of growing on a multi-carbon substrate as a primary or sole carbon source and methods for growing methanotrophic bacteria on a multi-carbon substrate.

主权项

1. A recombinant methanotrophic bacterium including at least one exogenous nucleic acid encoding a multi-carbon substrate utilization pathway component,
wherein the multi-carbon substrate is not utilized as a carbon source by a reference methanotrophic bacterium; wherein the at least one exogenous nucleic acid encoding a multi-carbon substrate utilization pathway component is expressed in a sufficient amount to permit growth of a recombinant obligate methanotrophic bacterium on the multi-carbon substrate as a primary carbon source or a recombinant facultative methanotrophic bacterium on the multi-carbon substrate as a sole carbon source.