Passive Replacement of Media

摘要

Embodiments described herein generally relate to passively replacing media in a closed cell expansion system to reduce or prevent the dilution of chemical signaling used to inhibit signaling pathways that keep a cell population in the lag phase of cell growth. To prevent such dilution, active inlet fluid flow to the system may be halted. To replace fluid lost by the system, a bag containing media may be attached to the waste line in replacement of the waste or outlet bag connected thereto. By turning off one or more pumps, media from the replacement bag is added to the system at the rate of evaporation. Chemical signaling dilution may be prevented while conserving system resources. Enhancement of chemical signaling to reduce the lag phase of cell growth may further be accomplished by adding molecules, such as chemical-signaling proteins, from a direct source to the system.

主权项

1. A method for controlling chemical signaling in a bioreactor of a closed cell expansion system, the method comprising:
coating the bioreactor; loading cells into the bioreactor, wherein the cells are loaded from a cell inlet bag into the bioreactor; activating an intracapillary circulation pump; replacing a waste bag attached to the cell expansion system with a media bag; and after the replacing the waste bag with the media bag, deactivating the intracapillary circulation pump.