发明名称 |
ISOTHERMAL AMPLIFICATION UNDER LOW SALT CONDITION |
摘要 |
Provided herein are methods and kits for isothermal nucleic acid amplifications that use a target nucleic acid template; a reaction mixture comprising a DNA polymerase having a strand displacement activity, a deoxyribonucleoside triphosphate (dNTP) mixture, a primer with a 3' end and a 5' end, a molecular crowding reagent, and a buffer solution for amplifying the target nucleic acid template. The buffer solution maintains a low salt concentration of the reaction mixture, and wherein the salt concentration results in a melting temperature (Tm) of the primer at least 10 C below the reaction temperature. The amplification is effected under isothermal condition. |
申请公布号 |
CA2939282(A1) |
申请公布日期 |
2015.10.01 |
申请号 |
CA20152939282 |
申请日期 |
2015.03.18 |
申请人 |
GENERAL ELECTRIC COMPANY |
发明人 |
HELLER, RYAN CHARLES;NELSON, JOHN RICHARD;PATEL, PARESH LAKHUBHAI;WAKEFIELD, ALISON MYFANWY;CAPPER, STEPHEN JAMES |
分类号 |
C12Q1/68;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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