MEANS AND METHODS FOR THE ENZYMATIC PRODUCTION OF L-METHIONINE FROM O-PHOSPHO-L-HOMOSERINE AND METHANETHIOL

摘要

Provided is a method for producing L-methionine in which O-phospho-L-homoserine and methanethiol are enzymatically converted into L-methionine and H3PO4. Such a conversion is achieved by an enzyme called O-phospho-L-homoserine (OHPS) dependent methionine synthase. Also described are O-phospho-L-homoserine (OHPS) dependent methionine synthases, i.e. proteins which are able to enzymatically convert O-phospho-L-homoserine and methanethiol into L-methionine and H3PO4 as well as microorganisms which have been genetically modified so as to be able to produce L-methionine from O-phospho-L-homoserine and methanethiol. Furthermore described are methods to screen for enzymes that catalyse the conversion of O-phospho-L-homoserine and methanethiol into L-methionine and H3PO4.

主权项

1. A method for the production of L-methionine in which O-phospho-L-homoserine and methanethiol are enzymatically converted into L-methionine and H3PO4 according to the following reaction scheme:
O-phospho-L-homoserine+CH3—SH<=>L-methionine+H3PO4.