METHOD FOR RECOMBINANT PRODUCTION OF LABYRINTHOPEPTINS AND FUNCTIONAL DERIVATIVES THEREOF

摘要

The present invention relates to a method for recombinant production of Labyrinthopeptins and functional derivatives thereof. Moreover, the present invention relates to novel functional derivatives of Labyrinthopeptins.

主权项

1. A nucleic acid comprising at least one Labyrinthopeptin (lab) gene cluster comprising a sequence according to SEQ ID No. 139, wherein:
(a) the nucleic acid further comprises a constitutive promoter for expression in Streptomyces cells and wherein the gene cluster is under control of said promoter, or (b) the nucleic acid encoding LabA1 or LabA2 or a functional derivative thereof is mutated, such that the C-terminal amino acid of the leader peptide of LabA1 or LabA2 is substituted with a methionine, or (c) the nucleic acid further comprises at least one hybrid gene sequence, wherein the hybrid gene sequence:
(i) encodes a pre-pro-peptide comprising a LabA2 leader sequence and LabA1 pre-peptide or functional derivative thereof and/or(ii) encodes a pre-pro-peptide comprising a LabA1 leader sequence and LabA2 pre-peptide or functional derivative thereof.