Collagen-Derived Recombinant Proteins with Von Willebrand Factor-Binding Activity

摘要

A method for diagnosing von Willebrand disease and novel polypeptides which bind to von Willebrand factor.

主权项

1) A method for diagnosing von Willebrand disease in a patient, comprising the following steps:
a) Providing a polypeptide selected from:
the polypeptide comprising the sequence from position 24 toposition 184 of SEQ ID NO: 2,the polypeptide comprising the sequence from position 24 to position 205 of SEQ ID NO: 4,the polypeptide comprising the sequence from position 24 to position 226 of SEQ ID NO: 5,a von Willebrand factor-binding polypeptide comprising the peptide motif Z1-GPRGQPGVMGFP-Z2-GPRGQPGVMGFP and the peptide motif (GPP)k wherein independentlyZ1 represents a linker comprising 6 to 12 amino acids,Z2 represents a linker comprising 6 to 12 amino acids,k is an integer between 4 and 15; b) Contacting a biological sample previously taken from the patient with said polypeptide; c) Measuring the binding of von Willebrand factor present in the biological sample to the polypeptide of step a) in order to measure von Willebrand factor collagen-binding activity.