METHOD FOR SUPPRESSING DESIALYLATION OF SUGAR CHAIN IN PREPARATION OF LABELED SUGAR CHAIN SAMPLE

摘要

This method for suppressing desialylation of a sugar chain in the preparation of a labeled sugar chain sample comprising a sugar chain including terminal sialic acid comprises: (a) a step for capturing sugar chains using polymer particles by bringing a sample containing sugar chains having terminal sialic acid into contact with polymer particles having functional groups for capturing sugar chains; (b) a step for washing the polymer particles that have captured the sugar chains; (c) a step for causing the sugar chains to separate from the polymer particles that have trapped the sugar chains; and (d) a step for labeling the separated sugar chains. The reaction of at least one of steps (a), (c) and (d) is performed under conditions of 0 to 60˚C for 10 seconds to 24 hours.