RNA-BASED, AMPLIFICATION-FREE, ORGANISM IDENTIFICATION USING NANO-ENABLED ELECTRONIC DETECTION

摘要

A technique that uses nanotechnology to electrically detect and identify RNA sequences without the need for using enzymatic amplification methods or fluorescent labels. The technique may be scaled into large multiplexed arrays for high-throughput and rapid screening. The technique is further able to differentiate closely related variants of a given bacterial or viral species or strain. This technique addresses the need for a quick, efficient, and inexpensive bacterial and viral detection and identification system.

主权项

1. A method for identifying organisms, the method comprising:
providing a target RNA molecule from an organism; contacting, under hybridizing conditions, the target RNA molecule with a complimentary nucleic acid probe, said probe attached to a nanostructured electrode, wherein hybridization of the target RNA molecule and complimentary nucleic acid probe provides an electrochemical current; measuring said electrochemical current; and identifying the organism based on said electrochemical current measurement.