METHODS, KITS, AND SYSTEMS FOR CLARIFYING PIGMENTED SAMPLES

摘要

Methods, kits, and systems for clarifying biological samples containing obfuscating pigment are disclosed. An automated method of treating a sample mounted on a substrate to alleviate staining obfuscations associated with pigments within the sample includes applying a clarifying reagent so that the clarifying reagent contacts the sample and the pigments within the sample are decolorized. The pigments within the sample being decolorized enable the specifically stained sample to be interpretable by a qualified reader.

主权项

1. An automated method of treating a sample mounted on a substrate to alleviate staining obfuscations associated with pigments within the sample, the method comprising the steps of:
placing the substrate, upon which the sample is mounted, on an automated instrument, applying a clarifying reagent in an amount sufficient to contact the sample and decolorize the pigments in said sample, applying a rinsing reagent so that the clarifying reagent is substantially removed from contacting the sample, and applying a chromogenic reagent so that the sample is specifically stained;wherein the pigments within the sample are decolorized so that the specifically stained sample is interpretable by a qualified reader.