DETECTION OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS

摘要

The present methods pertain to amplifying and/or detecting Staphylococcus aureus (“SA”) and methicillin-resistant Staphylococcus aureus (“MRSA”) nucleic acids based on a combined detection of ldh1 as a SA marker and mecA as a MRSA marker. In certain embodiments the methods also pertain to amplifying and/or detecting one or more SCCmec integration sites or bridge regions. Primers and probes are suitable to be used in the present methods to detect SA and MRSA simultaneously in a single reaction or in separate reactions. The amplified nucleic acid can be detected by a variety of state of the art methods, including fluorescence resonance energy transfer (“FRET”), radiolabels, enzyme labels, and the like.

主权项

1. A method for detecting a methicillin-resistant Staphylococcus aureus (“MRSA”) in a sample containing nucleic acids, comprising:
amplifying the nucleic acids in the sample; and detecting nucleic acids in the sample comprising amplified mecA and ldh1 genes, wherein the presence of amplified mecA and ldh1 genes indicates the presence of methicillin-resistant Staphylococcus aureus, and wherein the amplified mecA and ldh1 genes are present in approximately 1:1 ratio.