EXPRESSION METHOD OF ACTIVE GOLDFISH TGF2 TRANSPOSON RECOMBINANT TRANSPOSASE PROTEIN

摘要

An expression method of active goldfish gfTP1 transposase protein comprises: constructing an expression vector comprising a gfTP1 transposase reading frame of a goldfish Tgf2 transposon, after transferred into escherichia coli Rosetta 1(DE3), culturing an expression strain until absorbance of a bacteria solution under OD600 reaches 0.3-0.4, adding IPTG, culturing under 21-22℃ in shaking of 150-200rpm, inducing to express soluble recombinant protein, and purifying to obtain a functionally active transposase. Also provided are an expression plasmid and the expression strain of the goldfish gfTP1 transposase protein, and a use of the strain in transgenosis.