METHOD FOR PREPARING HOMODIMER PROTEIN MIXTURE BY USING CHARGE REPULSION EFFECT

摘要

The invention relates to a method for preparing a homodimer protein mixture by using repulsive interaction of charges. The method comprises the step of replacing part of residues with the opposite-charged residues, so that different proteins or antibodies are unfavorable to forming heterodimers due to the repulsive interaction between like charges, while same proteins or antibodies are favorable to forming homodimers due to attractive interaction between opposite charges. The homodimer protein mixture obtained according to the method of the invention can simultaneously act on different epitopes of the same target, and simultaneously inhibit the effects of a plurality of antigens by binding to the antigens from different sources, thereby providing a new approach towards immunological diagnosis and treatment of tumors and other diseases.

主权项

1. A method for obtaining a mixture containing two or more proteins (such as antibodies) by using single recombinant cell clone, wherein the proteins is in the form of a dimer which is formed by polymerization between monomer chains, and the two or more proteins contain the same domain, wherein the method comprises the step of replacing part of amino acid residues of the two monomer chains in the same domain of one or more proteins with the opposite-charged residues, so that the monomer chains from different proteins are unfavorable to forming a heterodimer due to the repulsive interaction between like charges, while the monomer chain from the same protein is more favorable to forming a homodimer due to the attractive interaction between opposite charges.