| 摘要 |
<p>A method for identifying a biomarker indicative of a reduced response to a drug in a patient comprising the steps of a) heating a sample from a patient treated with the drug and responsive to the drug, b) separating soluble from insoluble protein in the product of step a), c) analysing either or both the soluble and insoluble protein fractions of step b), d) repeating steps a) to c) with a sample from a patient treated with the drug wherein said patient has a reduced response to the drug, and e) identifying at least one protein which has a different melting temperature in the sample from the drug responsive patient compared to the sample from the drug non-responsive patient, wherein said protein is a biomarker indicative of a reduced response to the drug. Also claimed is a method for determining drug responsiveness in a patient comprising analysing a heated sample for the presence of a target protein; a method for determining the activation state of a protein in a sample comprising analysing a heated sample for the presence of the protein and a method for investigating the structural identity between a recombinantly produced drug and an approved drug comprising identifying the difference in melting temperature of the drugs.</p> |
