SELECTION FREE GROWTH OF HOST CELLS CONTAINING MULTIPLE INTEGRATING VECTORS

摘要

The present invention relates to the production of proteins in host cells, and more particularly to host cells containing multiple integrated copies of an integrating vector comprising an exogenous gene. The present invention further relates to the use of integrating vectors lacking a selectable marker and growth of host cells containing such vectors in the absence of selection. The present invention further provides methods of expressing increased levels of protein in host cells using such vectors.