It is intended to provide a freeze-tolerant yeast. It is also intended to provide a frozen dough which shows an enough rise when fermented after the freezing and a bread made from this frozen dough. Moreover, it is intended to provide a method by which the freeze-tolerant yeast as described above is obtained. A yeast is cultured in a medium containing a protein digest. By using the thus obtained yeast, a frozen dough and a bread made from the frozen dough are produced.