发明名称 |
METHOD FOR DECONVOLVING SINGLE-MOLECULE INTENSITY DISTRIBUTIONS FOR QUANTITATIVE BIOLOGICAL MEASUREMENTS |
摘要 |
A method for quantifying fluorescent puncta comprising acquiring at least one first intensity distribution comprising fluorescence intensity values from a plurality of first fluorescent puncta; acquiring at least one second intensity distribution comprising fluorescence intensity values from a plurality of second fluorescent puncta, wherein each second fluorescent puncta has a determined number of fluorescent emitters; determining the relationship between the first and second intensity distributions; and fitting the second intensity distribution to the first intensity distribution to provide a count and distribution of the number of fluorescent emitters within the first fluorescent puncta.
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申请公布号 |
US2008052009(A1) |
申请公布日期 |
2008.02.28 |
申请号 |
US20070844250 |
申请日期 |
2007.08.23 |
申请人 |
WASHINGTON, UNIVERSITY OF |
发明人 |
CHIU DANIEL T.;NIELSEN SARAH A.;FUJIMOTO BRYANT S.;KUYPER CHRISTOPHER L.;KUO JASON S.;BAJJALIEH SANDRA M. |
分类号 |
G01N33/48 |
主分类号 |
G01N33/48 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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