<p>Disclosed is an RNA detection method wherein a target RNA chain derived from a target sample is detected from a reaction system containing the target sample by using a substrate whose surface contains a polymer substance containing a first unit having a group derived from a phosphate ester constituting the hydrophilic portion of a phospholipid and a second unit having a carboxylic acid derivative group obtained by bonding an electron-withdrawing substituent to a carbonyl group, while being provided with at least one reaction space in which an immobilized DNA primer is contained.</p>
