| 摘要 |
<p>The present invention relates to an“in vitro”diagnostic method for diseases affecting human or animal tissues, in particular for the diagnosis of diseases involving inflammation and fibrosis in human or animals, more particularly for liver diseases. More particularly, the present invention relates to a method for diagnosing“in vitro”abnormal morphological conditions in human or animal tissues affected by a chronic inflammatory disease, which comprises observing an image of a biopsy sample of the human or animal body in which said abnormal condition can be detected and metrically quantifying said abnormal morphological condition, wherein said step of metrically quantifying comprises detecting the extent of the fibrotic and of the inflammatory tissue by means of: i) calculating the fractal corrected perimeter (Pf) and/or area (Af) of the collagen islets, and ii) calculating the percentage area of the clustered inflammatory cells by means of the formula ACINF/AB·100, wherein ACiNF is the actual area of the inflammatory cells belonging to clusters and AB is the area of the biopsy sample.</p> |
