发明名称 |
METHOD FOR PREPARING FISH EMBRYO |
摘要 |
<p>An object of the invention is to effectively prepare a fish embryo with a correct chromosomal ploidy by nuclear transplantation in which an exogenous fish nucleus is transplanted in a cytoplasmic recipient. For this object, the invention comprises a step of preparing a fish embryo by transplanting a fish cell nucleus to an unfertilized egg. The step of preparing a fish embryo comprises a step of imposing physical and/or chemical stress to the unfertilized egg after activation. By imposing such stress, the stage of haplosis in a female nucleus which happens at the early stage of a series of developmental steps occurring in an unfertilized egg is suppressed and the correct ploidy of an obtained embryo is at least secured.</p> |
申请公布号 |
WO2007096985(A1) |
申请公布日期 |
2007.08.30 |
申请号 |
WO2006JP303447 |
申请日期 |
2006.02.24 |
申请人 |
NATIONAL UNIVERSITY CORPORATION NAGOYA UNIVERSITY;WAKAMATSU, YUKO;OZATO, KENJIRO |
发明人 |
WAKAMATSU, YUKO;OZATO, KENJIRO |
分类号 |
A01K67/02;A01K67/027;C12N5/10;C12N15/09 |
主分类号 |
A01K67/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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