发明名称 |
METHODS OF INCREASING EXPRESSION LEVELS OF HUMAN TNF RECEPTOR |
摘要 |
<p>The present invention relates to methods of increasing protein expression levels whereby at least one amino acid in a protein amino acid sequence is substituted for the amino acid, proline. Preferably, the substitution occurs within 15 amino acids, more preferably within 10 amino acids and most preferably within 5 amino acids of a cysteine amino acid residue. The present invention not only includes methods for polypeptides with proline substitutions, but also polynucleotides with codon substitutions for which a codon for any amino acid, except proline, is substituted for a codon encoding for proline.</p> |
申请公布号 |
EP1448772(B1) |
申请公布日期 |
2007.07.18 |
申请号 |
EP20020777356 |
申请日期 |
2002.11.21 |
申请人 |
APPLIED RESEARCH SYSTEMS ARS HOLDING N.V. |
发明人 |
BRONDYK, WILLIAM;JIANG, XULIANG;SCHWEICKHARDT, RENE, LYNN |
分类号 |
C12N15/00;C12N15/09;C07K14/47;C07K14/715;C12N15/11;C12P21/02 |
主分类号 |
C12N15/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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