METHOD FOR DECIDING MATURITY USING POLYCLONAL ANTIBODY OF ROCKFISH

摘要

A method for diagnosis of maturity by using a polyclonal antibody of rockfish(Sebastes schlegeli) is provided to improve diagnosis accuracy of rockfish maturity by measuring the amount of luteinizing hormone(LH) in rockfish. The method for diagnosis of maturity by using a polyclonal antibody of rockfish(Sebastes schlegeli) comprises the steps of: collecting the pituitary gland of rockfish and purifying it to obtain the rockfish luteinizing hormone; separating rockfish glycoprotein alpha(GPalpha) and rockfish luteinizing hormone beta(LHbeta) from the rockfish luteinizing hormone, and independently obtaining polyclonal antibodies against the rockfish glycoprotein alpha(GPalpha) and rockfish luteinizing hormone beta(LHbeta); and deciding maturity of rockfish through enzyme-linked immunoassay method by using the polyclonal antibody against rockfish luteinizing hormone beta(LHbeta).