| 摘要 |
A luminous microorganism immobilized in microsomes, a manufacturing method thereof and a monitoring method of poisonous materials by using the same microorganism are provided to enhance sensitivity of the monitoring by reducing the size of microsomes, and improve monitoring accuracy by minimizing the errors caused by a control group. The luminous microorganism immobilized in microsomes having diameter of 0.01-0.03 mm is provided, wherein the luminous microorganism is Vibrio fischeri, and shows reduced luminescence in proportion to concentration of poisonous materials in a sample; and the immobilization material is alginic acid, agar, carrageenan or polyacrylamide. The method for manufacturing the luminous microorganism immobilized in microsomes comprises the steps of: mixing the luminous microorganism with the immobilization material, and culturing the mixture in seawater complete medium at 22-27 deg. C for 45-50 hours; suspending the recovered luminous microorganism cells with 0.5-0.8% physiological saline solution, centrifuging the cells and resuspending them with 2-3% physiological saline solution; and mixing the suspended microorganism solution with the immobilization material, adding dropwise of the mixture to calcium salt or strontium salt solution, and maintaining the solution at 0-5 deg. C for 1-3 hours to obtain gel type microbeads.
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