ELECTROCHEMICAL ASSAY

摘要

A method of determining the presence or amount of analyte in a fluid sample, which comprises: (a) contacting a fluid sample with a binding reagent that comprises a plurality of cleavable species and wherein said species, when cleaved, are detectable using electrochemical means; (b) separating any binding reagent-analyte complex that forms from the unbound binding reagent; (c) cleaving the cleavable species from the immobilized binding reagent- analyte complex; and (d) detecting the cleaved species using electrochemical means.