PROCESSES FOR ADENOVIRUS PURIFICATION USING CONTINUOUS FLOW CENTRIFUGATION

摘要

The present invention relates to methods for the scalable preparation for adenoviral preparations comprising the steps of: culturing host cells comprising adenovirus; obtaining supernatants from the host cells; applying said supernatants to a centrifugal apparatus comprising a 50% w/v solution of non-ionic gradient; applying centrifugal force to said supernatants such that the flow is continuous and directed from bottom-to-top; separating the adenoviral particles according to their density; and obtaining high-yield fractions comprising active adenoviral particles.