发明名称 |
Method to increase class I presentation of exogenous antigens by human dendritic cells |
摘要 |
The present invention relates to a method for increasing the uptake, processing and class I presentation of a soluble antigen by an immature dendritic cells, comprising the steps of:
a) obtaining a population comprising human immature dendritic cells;
b) culturing the population immature dendritic cells in vitro in a cell culture medium supplemented with a agent that promotes Major-Histocompatability Complex - (MHC-) class I processing and a soluble antigen associated with a tumor cell for a time period sufficient for antigen uptake, processing and presentation, and until maturation of the immature dendritic cells in the population;
c) separating the soluble tumor antigen and the agent that promotes MHC-class I processing from the mature tumor antigen presenting dendritic cells; and
d) formulating the mature tumor antigen presenting dendritic cells for administration to a patient. |
申请公布号 |
AU2001259760(B2) |
申请公布日期 |
2006.10.05 |
申请号 |
AU20010259760 |
申请日期 |
2001.05.11 |
申请人 |
NORTHWEST BIOTHERAPEUTICS, INC. |
发明人 |
MICHAEL L. SALGALLER;ALTON L. BOYNTON |
分类号 |
A61K39/00;C12N5/06;A61K35/12;A61K35/14;A61K35/24;A61K35/26;A61K35/28;A61K35/36;A61K35/50;A61P35/00;A61P35/04;A61P37/04 |
主分类号 |
A61K39/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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