Packing cell system for eb virus vector

摘要

A packaging cell is constructed by preparing a packaging cell carrying an EB virus gene lacking a packaging signal but not carrying a wild type EB virus gene having the packaging signal, and introducing an amplicon plasmid having the packaging signal but having no viral replication ability into the cell. By inducing lytic infection of the packaging cell introduced with an amplicon plasmid, an EB virus vector without viral replication ability is produced.