A method for simultaneous production of multiple proteins; vectors and cells for use therein

摘要

A cell comprises two polypeptide expression units each encoding at least one polypeptide of interest, characterized in that the polypeptide units each comprise at least one STAR (stabilizing anti-repressor) sequence having the capacity to at least in part block chromatin-associated repression and is selected from the group consisting of: (a) any one of SEQ ID:1 through SEQ ID:65; (b) a functional equivalent of any one of SEQ ID:1 through SEQ ID:65; and (c) a functional fragment of any one of SEQ ID:1 through SEQ ID:65. Also described are polypeptide expression units, methods to obtaining a host cell expressing the two polypeptides of interest, and methods for expressing the two polypeptides of interest.