METHOD OF SEPARATING PROTEIN, METHOD OF STAINING PROTEIN AND LIQUID PROTEIN-STAINING AGENT AND PROTEIN-STAINING KIT TO BE USED IN THESE METHODS

摘要

A method of separating a protein by the gel electrophoresis, which comprises the staining step of contacting a liquid staining agent containing a staining agent and a surfactant in a buffer solution with a sample containing the protein, and the electrophoresis step of electrophoresing in a gel the protein-containing sample having been stained in the above step. According to this method, a protein-containing sample can be stained within a short period of time by using a liquid staining agent containing a surfactant such as SDS and the color development can be achieved at a high sensitivity. Moreover, the excessive staining agent migrates before the protein in the electrophoresis, which makes it unnecessary to employ any washing operation. It is also possible that a sample is stained with a staining agent containing a surfactant such as SDS after the one-dimensional electrophoresis and then immediately subjected to the two-dimensional electrophoresis. Thus, the number of steps can be reduced compared with the existing protein separation methods and the separation procedure can be simplified. Namely, a method of conveniently and quickly separating a protein by the electrophoresis can be provided.