发明名称 |
BIOSYNTHETIC PRODUCTION OF 4-AMINO-4-DEOXYCHORISMATE (ADC) AND [3R,4R]-4-AMINO-3-HYDROXYCYCLOHEXA-1,5-DIENE-1-CARBOXYLIC ACID (3,4-CHA) |
摘要 |
The invention relates to a process for the biosynthetic production of 4-amino-4-deoxychorismate (ADC) performed fermentativelyin vivo with a 4-amino-4-deoxychorismate synthase, preferably a PabAB bipartite protein (which may be a fusion protein), at an increased level of activity, thereby obtaining a broth comprising ADC and 4-amino-4-deoxyprephenate (ADP), that are recovered. The invention also relates to a further process of converting the ADP into p-aminophenylalanine. The invention, moreover relates to biosynthetic production of [3R,4R]-4-amino-3-hydroxycyclohexa-1,5-diene-1-carboxylic acid (3,4-CHA), by concerted action of such 4-amino-4-deoxychorismate synthase and of an enzyme capable of converting isochorismate into [5S,6S]-5,6 dihydroxycyclohexa-1,3-diene-1carboxylic acid (2,3-CHD), preferably a phenazine biosynthesis protein PhzD, including recovery of 3,4-CHA. The invention also relates to expression vectors and host cells for use in any of such processes. The invention further relates to the use of 3,4-CHA as a catalytically active product, in particular as a chiral catalyst. And the invention finally relates to synthesis of oseltamivir phosphate from 3,4-CHA. |
申请公布号 |
WO2005118829(A3) |
申请公布日期 |
2006.06.15 |
申请号 |
WO2005EP05988 |
申请日期 |
2005.06.02 |
申请人 |
DSM IP ASSETS B.V.;DSM BIOTECH GMBH;FORSCHUNGSZENTRUM JUELICH GMBH;WUBBOLTS, MARCEL, GERHARDUS;BOVENBERG, ROELOF, ARY, LANS;SPRENGER, GEORG;BONGAERTS, JOHANNES, JOSEF;KOZAK, STEFAN;MUELLER, MICHAEL |
发明人 |
WUBBOLTS, MARCEL, GERHARDUS;BOVENBERG, ROELOF, ARY, LANS;SPRENGER, GEORG;BONGAERTS, JOHANNES, JOSEF;KOZAK, STEFAN;MUELLER, MICHAEL |
分类号 |
C12P13/00;C07C229/48;C12N1/14;C12N1/20;C12P7/42;C12P13/04 |
主分类号 |
C12P13/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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