发明名称 |
DNA CHAIN ELONGATION METHOD, DNA CHAIN AMPLIFICATION METHOD AND MICROARRAY FOR DNA CHAIN ELONGATION |
摘要 |
<p>A primer for DNA elongation is immobilized to a substrate having a polymer, which comprises a first unit having a group induced from a phosphate constituting the hydrophilic moiety of a phospholipid and a second unit having an active ester group, on its surface. Then, a reaction system, into which a template DNA fragment having a desired sequence and a sample containing a nucleotide monomer have been introduced, is heated to a temperature at which the DNA chain is thermally denatured. Next, the temperature of the above reaction system is lowered to a temperature for performing an annealing treatment and the DNA chain elongation is carried out in this reaction system.</p> |
申请公布号 |
WO2006062009(A1) |
申请公布日期 |
2006.06.15 |
申请号 |
WO2005JP21922 |
申请日期 |
2005.11.29 |
申请人 |
SUMITOMO BAKELITE CO., LTD.;DNA CHIP RESEARCH INC.;KINOSHITA, KENJI |
发明人 |
KINOSHITA, KENJI |
分类号 |
C12N15/09;C12M1/00;G01N33/53;G01N37/00 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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