| 摘要 |
A pipetting apparatus ( 1 ) comprises a fluidic space ( 7 ), to which a pressure transducer ( 11 ) with a pressure sensor ( 12 ) is attached with a gas filled space ( 15 ). The fluidic space ( 7 ) is defined by a pipette tip ( 2 ), a first tubing ( 5 ) that connects the pipette tip ( 2 ) to a pump ( 4 ), and an active part ( 6 ) of the pump ( 4 ). The pipetting apparatus ( 1 ) according to the present invention is characterized in that the pipetting apparatus ( 1 ) further comprises an impulse generating means ( 16,18,19 ) that is in operative contact with a column ( 10 ) of system liquid ( 8 ) inside the fluidic space ( 7 ). The impulse generating means ( 16,18,19 ) is designed to induce a vertical movement in this system liquid column ( 10 ), which results in a pressure variation in the gas filled space ( 15 ) that is pneumatically connected with the fluidic space ( 7 ). This pressure variation-as recorded with the pressure transducer ( 11 ) and as processed by a first data processing unit ( 13 ) during utilization of this pipetting apparatus-is taken as an indicator for the detection of penetration or of quitting of a surface ( 17 ) of a liquid, with an orifice ( 3 ) of the pipette tip ( 2 ), of which liquid an amount is to be aspirated and dispensed. This pressure variation is also taken as an indicator for the detection of the presence or the absence of gas bubbles in the system liquid ( 8 ) contained in the fluidic space ( 7 ) of this pipetting apparatus.
|
