IMPROVED RECOVERY OF ANALYTES FOR PROCESS CHROMATOGRAPHY

摘要

The present invention provides for a process scale or preparative scale chromatographic method of purifying biological material comprising the steps of providing chromatographic media including inorganic material in the form of porous particles; applying a solution comprising the biological material to the media, wherein the biological material is reversibly bonded to the media; and eluting the biological material from the media with a solvent, wherein the porous particles are subjected to a pretreatment in order to reduce BET surface area.