| 摘要 |
Protein detection microarrays are used to specifically detect cytokines and growth factors that are associated with wound healing and with host organism responses to foreign, implanted materials (i.e., biocompatibility). Methods of establishing profiles that can be used in evaluating wound healing and biocompatibility take advantage of such cytokine- and growth factor-specific microarrays, which comprise anti-cytokine and anti-growth factor capture antibodies immobilized onto a solid substrate. The microarrays are produced using a printing buffer optimized for cytokine/growth factor detection. Methods of detecting the cytokines and growth factors also utilize optimized blocking buffers, fluorescent dyes, and immunoassay conditions. Sandwich and direct label fluoroimmunoassays can be carried out with the optimized microarrays. Kits for establishing profiles that can be used in the evaluation of wound healing and biocompatibility comprise cytokine- and growth factor-specific microarrays, and optionally include buffers suitable for detection immunoassays, including optimized printing buffers and blocking buffers.
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