DELTA-ENDORPHIN

摘要

PURPOSE:To obtain a recombinant plasmid pENDD2 being a recombinant plasmid having 4682 pair of bases and capable of producing delta-endorphin of morphine like peptide having high analgesic activity when Escherichia coli is transformed by the above-mentioned plasmid. CONSTITUTION:Gene coding delta-endorphin expressed by the formula is synthesized and the synthesized gene is integrated into a plasmid pMEK2 to prepare a fused gene (pENDCD2) of delta-endorphin gene and dihydrofolic acid reducing enzyme (DHFR) gene, by which Escherichia coli is then transformed. DHFR-delta- endorphin fused protein is produced by culturing the transformed Escherichia coli and then the fused protein is digested by a proteolytic enzyme to separate delta-endorphin.