摘要 |
PURPOSE:To stably separate and collect a triglyceride with a high alpha-linolenic acid content in a high concentration by fractionating alpha-linolenic acid-containing natural fats and coils according to chromatography using a supercritical liquid, etc., as a mobile phase. CONSTITUTION:Natural fats and oils, such as perilla oil, containing alpha-linolenic acid, are fractionated by chromatography using a fluid or liquefied gas, such as CO2, in a supercritical state as a mobile phase [preferred conditions; without using an entrainer under 190-900kg/cm<2> pressure (at the column inlet)] to separate and collect a triglyceride fraction with a high alpha-linolenic acid content (e.g. 98%). |