发明名称 |
METHOD FOR DETECTION OF HUMAN IMMUNODEFICIENCY VIRUS AND CELL LINES USEFUL THEREFOR |
摘要 |
A method provided for assaying for human immunodeficiency virus comprises: seeding dishes with cells susceptible to HIV infection; adding to the dishes a sample to be tested for HIV infection; incubating the cells; exposing the cells to a first antibody reactive with HIV proteins; exposing the cells to marker-conjugated antibody reactive with the first antibody; examining the dishes with a suitable microscope; and counting the number of foci of marker-positive cells per dish to determine the number of infected cells in the dish. The process can be used to detect and to quantify HIV-infected cells, which permits monitoring of the effects of drug therapy on the levels of HIV expression in AIDS patients, as well as providing a quantitative measure of HIV expression in asymptomatic HIV antibody-positive individuals. |
申请公布号 |
WO9112337(A1) |
申请公布日期 |
1991.08.22 |
申请号 |
WO1991US00956 |
申请日期 |
1991.02.11 |
申请人 |
THE UNITED STATES OF AMERICA, REPRESENTED BY THE S |
发明人 |
CHESEBRO, BRUCE, WILCOX;WEHRLY, KATHRYN, ANN |
分类号 |
C12N5/10;C12N15/09;C12R1/91;G01N33/536;G01N33/569 |
主分类号 |
C12N5/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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