发明名称 |
Method of measuring glycolated hemoglobin A1C, enzyme to be used therefor and process for producing the same |
摘要 |
There is provided a method for specifically determining a glycated ²-chain N-terminal of glycated hemoglobin using enzymes without a separation operation, and a determination reagent kit therefor. A protease that cleaves a glycated amino acid and/or a glycated peptide from a glycated ²-chain N-terminal without substantially cleaving a glycated amino acid or a glycated peptide from a glycated ±-chain N-terminal of glycated hemoglobin or a fragment thereof is screened. The method of specifically determining a glycated ²-chain N-terminal of glycated hemoglobin and the determination reagent kit are provided by using the protease obtained by the screening method. According to the present invention, a glycated ²-chain N-terminal of glycated hemoglobin can specifically be determined without a separation operation. |
申请公布号 |
EP2096173(A1) |
申请公布日期 |
2009.09.02 |
申请号 |
EP20090004586 |
申请日期 |
2004.05.21 |
申请人 |
ASAHI KASEI PHARMA CORPORATION;ICHIBIKI CO LTD. |
发明人 |
MATSUOKA, TAKESHI;KOGA, SHINJI;KOUZUMA, TAKUJI;YOSHIOKA, ISSEI;NISHIMURA, ATSUHISA;ITUO, HOMARE;KUMAZAWA, TOSHIHIKO;KUROYANAGI, TAKASHI |
分类号 |
C12N15/53;C12N1/20;C12N1/21;C12N9/02;C12N9/50;C12Q1/34;C12Q1/37;G01N33/15;G01N33/50;G01N33/66;G01N33/72 |
主分类号 |
C12N15/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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