METHOD FOR PREPARING NOVEL MUTATED AND MULTI-MUTATED BRAZZEIN HAVING HIGHER SWEETNESS

摘要

A novel multi-mutated Brazzein having high sweetness is provided to improve sweetness by maintaining pH stability and replace or mix to more various sweetner. A method for producing a multi-mutated Brazzein comprises: a step of culturing E.coli which is transformed with mutant gene which encodes E.coli pelB signal sequence and the mutated Brazzein; a step of isolating a protein of cell membrane of E.coli; and a step of heating the protein of cell membrane.