Method of quantifying a substrate

摘要

A method of measuring a quantity of a substrate contained in sample liquid is provided. This method can reduce measurement errors caused by a biosensor. The biosensor includes at least a pair of electrodes on an insulating board and is inserted into a measuring device which includes a supporting section for supporting detachably the biosensor, plural connecting terminals to be coupled to the respective electrodes, and a driving power supply which applies a voltage to the respective electrodes via the connecting terminals. One of the electrodes of the biosensor is connected to the first and second connecting terminals of the measuring device only when the biosensor is inserted into the measuring device in a given direction, and has a structure such that the electrode becomes conductive between the first and second connecting terminals due to a voltage application by the driving power supply.
The method comprising the steps of applying a first voltage to the electrodes during a first period; halting applying the voltage during a standby period after the first period; and measuring the quantity of the substrate by applying a second voltage to the electrodes during a second period after the standby period, the first voltage being greater than the second voltage.