Isolation of immunoglobulins

摘要

The present invention relates to a method for the isolation of immunoglobulins from a solution containing one or more immunoglobulins, comprising the following operations: a) contacting the solution containing one or more immunoglobulins having a pH in the range of 2.0 to 10.0 and a total salt content corresponding to an ionic strength of at the most 2.0 with a solid phase matrix comprising a functionalised matrix backbone carrying a plurality of functional groups of the following formula: M-SP1-L; wherein M designates the matrix backbone; SP1 designates a spacer; L designates a ligand comprising a mono- or bicyclic optionally substituted aromatic or heteroaromatic moiety; with the proviso that the molecular weight of the ligand -L is at the most 500 Dalton,
whereby at least a part of the immunoglobulins becomes bound to the solid phase matrix; and
b) separating the solid phase matrix having immunoglobulins bound thereto from the solution; and
c) optionally washing the solid phase matrix; and
d) contacting the solid phase matrix with an eluent in order to liberate one or more of the immunoglobulins from the solid phase matrix."