发明名称 |
QUANTITATIVE, MULTISPECTRAL IMAGE ANALYSIS OF TISSUE SPECIMENS STAINED WITH QUANTUM DOTS |
摘要 |
<p>The present invention relates to a method of whole slide imaging of a biological specimen, the specimen having 1 ... N fluorophores present in the specimen, at least one of which is a quantum dot, comprising the steps of:
(a) obtaining one or more images of the specimen with a digital camera at a low resolution;
(b) morphologically processing the one or more images or an image derived therefrom and automatically identifying one or more regions of interest containing biological structures present in the specimen;
(c) automatically obtaining an image cube of the one or more regions of interest with the camera at a high resolution, the image cube comprising image data for the regions of interest at M different wavelengths, where M is an integer greater than 2;
(d) determining from the image cube coefficients C 1 ... C N for each pixel imaging the one or more regions of interest, wherein the coefficients C 1 ... C N are related to the concentrations of the 1 ... N fluorophores present in the specimen imaged by each pixel;
(e) conducting a quantitative analysis of the regions of interest including calculating fluorophore concentrations for the biological structures from the coefficients C 1 ... C N ; and
(f) generating display data of the results of the quantitative analysis for display of a workstation.</p> |
申请公布号 |
EP2095094(A2) |
申请公布日期 |
2009.09.02 |
申请号 |
EP20070874167 |
申请日期 |
2007.12.07 |
申请人 |
VENTANA MEDICAL SYSTEMS, INC. |
发明人 |
LETT, G., SCOTT;HAUBEIN, NED, C.;PESTANO, GARY |
分类号 |
G01N21/64;G01N33/58;G06F19/00 |
主分类号 |
G01N21/64 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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