| 摘要 |
<P>PROBLEM TO BE SOLVED: To generate two living populations of dendritic cells of the skin and the mucous membranes, namely Langerhans cells (LC) and interstitial dendritic cells (IDC), in vitro from a single cellular precursor. <P>SOLUTION: Provided is the use of CD14+ monocytes for the production of dendritic cells. Provided is the use of CD14+ monocytes isolated from peripheral circulating blood for obtaining, by differentiation, at least one mixed population of Langerhans cells and interstitial dendritic cells, both Langerhans cells and interstitial dendritic cells being preconditioned and undifferentiated, and/or differentiated and immature, and/or mature, and/or interdigitated. Provided is their use in suspension, monolayer and three-dimensional cell and tissue models. Provided is the use of these cells and of these models as study models for the assessment of immunotoxicity/immunotolerance, for the development of cosmetic and pharmaceutical active principles and for the development and implementation of methods of cell and tissue therapy. <P>COPYRIGHT: (C)2009,JPO&INPIT |
