| 摘要 |
<p>The present invention relates to a novel protein having amidase activity that stereoselectively hydrolyzes alpha -amino acid amides and alpha -hydroxy acid amides, and a gene encoding said protein. Namely, the protein is (a) a protein containing an amino acid sequence of SEQ ID NO: 1, or (b) a protein containing the amino acid sequence of SEQ ID NO: 1 by deletion, replacement or addition of one or more amino acids and having amdiase activity that stereoselectively hydrolyzes alpha -amino acid amides and alpha -hydroxy acid amides. Thus, the present invention can provide a microorganism having multiple amidase genes cloned via gene recombination techniques in it cells, and, therefore, having a remarkably elevated catalytic capability compared with the conventional methods. As a result, optically active alpha -amino acid and alpha -hydroxy acid can be effectively produced from alpha -amino acid amides and alpha -hydroxy acid amides.</p> |
