发明名称 |
A PURE FORM OF RAPAMYCIN AND A PROCESS FOR RECOVERY AND PURIFICATION THEREOF |
摘要 |
<p>The present invention relates to a pure form of rapamycin with a total impurity content less than 1.2%; a process for recovery and purification of rapamycin comprising steps of (a) treating the fermentation broth, extracts or solutions containing rapamycin with water immiscible solvent and concentration; (b) addition of a water miscible solvent to effect separation of impurities present; (c) optionally, binding of the solvent containing the product from step (b) to an inert solid, washing the solid with a base and acid, followed by elution; (d) subjecting the elute from step (c) or the solvent containing the product from step (b) to silica gel chromatography; (e) crystallization of the product obtained from step (d); (f) subjecting a solution of the product from step (e) to hydrophobic interaction or reversed phase chromatography; and (g) re-crystallization to afford rapamycin in substantially pure form.</p> |
申请公布号 |
EP2079748(A1) |
申请公布日期 |
2009.07.22 |
申请号 |
EP20060842776 |
申请日期 |
2006.12.26 |
申请人 |
BIOCON LIMITED |
发明人 |
PATIL, NITIN SOPANRAO;HUSSAINI, SYED IDRIS;SINGH, ASHISH KUMAR;MENDHE, RAKESH BHAIYYARAM |
分类号 |
C07D498/18 |
主分类号 |
C07D498/18 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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